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ALL MEDICAL CODES IN CATEGORY Molecular Pathology Procedures

2025 CPT code 81273

KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (eg, mastocytosis), gene analysis, D816 variant(s).

2025 CPT code 81269

Analysis of HBA1/HBA2 genes for duplications and deletions, associated with alpha thalassemia.

2025 CPT code 81203

Analysis of the APC gene for duplication/deletion variants.

2025 CPT code 81301

Microsatellite instability analysis to detect mismatch repair deficiency, including comparison of neoplastic and normal tissue if performed.

2025 CPT code 81182

Analysis of the ATXN8OS gene to detect abnormal alleles, specifically expanded sequences, often associated with spinocerebellar ataxia 8 (SCA8).

2025 CPT code 81292

Complete gene sequencing analysis of the MLH1 gene.

2025 CPT code 81297

Analysis of the MSH2 gene for duplications and deletions.

2025 CPT code 81200

Analysis of the ASPA gene for common variants (e.g., E285A, Y231X).

2025 CPT code 81403

This CPT code represents the technical component of a Tier 2, Level 4 molecular pathology procedure.It encompasses various analyses of a single gene, including DNA sequencing, multiplex PCR, mutation scanning, or duplication/deletion variant analysis.

2025 CPT code 81296

Analysis of known familial variants in the MSH2 gene.

2025 CPT code 81111

Analysis of the common alleles of Human Platelet Antigen 9 (HPA-9) gene.

2025 CPT code 81229

Cytogenomic (genome-wide) microarray analysis for constitutional chromosomal abnormalities; interrogation of genomic regions for copy number and single nucleotide polymorphism (SNP) variants.

2025 CPT code 81355

Analysis of VKORC1 gene for common variants related to warfarin metabolism.

2025 CPT code 81210

BRAF (B-Raf proto-oncogene, serine/threonine kinase) gene analysis for V600 variant(s).

2025 CPT code 81383

HLA Class II typing, high resolution (ie, alleles or allele groups); one allele or allele group (eg, HLA-DQB1*06:02P), each

2025 CPT code 81121

IDH2 (isocitrate dehydrogenase 2 [NADP+], mitochondrial) (eg, glioma), common variants (eg, R140W, R172M).

2025 CPT code 81377

HLA Class II typing, low resolution (e.g., antigen equivalents); one antigen equivalent, each.

2025 CPT code 81272

KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (e.g., gastrointestinal stromal tumor [GIST], acute myeloid leukemia, melanoma), gene analysis, targeted sequence analysis (e.g., exons 8, 11, 13, 17, 18).

2025 CPT code 81238

F9 (coagulation factor IX) (eg, hemophilia B), full gene sequence

2025 CPT code 81230

Analysis of the CYP3A4 gene for common variants (e.g., *2, *22) to assess drug metabolism.

2025 CPT code 81247

G6PD (glucose-6-phosphate dehydrogenase) (eg, hemolytic anemia, jaundice), gene analysis; common variant(s) (eg, A, A-)

2025 CPT code 81329

SMN1 (survival of motor neuron 1, telomeric) (e.g., spinal muscular atrophy) gene analysis; dosage/deletion analysis (e.g., carrier testing), includes SMN2 (survival of motor neuron 2, centromeric) analysis, if performed.

2025 CPT code 81357

U2AF1 (U2 small nuclear RNA auxiliary factor 1) gene analysis, common variants (e.g., S34F, S34Y, Q157R, Q157P).

2025 CPT code 81360

ZRSR2 (zinc finger CCCH-type, RNA binding motif and serine/arginine-rich 2) (eg, myelodysplastic syndrome, acute myeloid leukemia) gene analysis, common variant(s) (eg, E65fs, E122fs, R448fs).

2025 CPT code 81372

HLA Class I typing, low resolution (e.g., antigen equivalents); complete (i.e., HLA-A, -B, and -C)

2025 CPT code 81379

HLA Class I typing, high resolution (ie, alleles or allele groups); complete (ie, HLA-A, -B, and -C)

2025 CPT code 81271

HTT (huntingtin) (e.g., Huntington disease) gene analysis; evaluation to detect abnormal (e.g., expanded) alleles

2025 CPT code 81316

PML/RARalpha,(t(15;17)), (promyelocytic leukemia/retinoic acid receptor alpha) (eg, promyelocytic leukemia) translocation analysis; single breakpoint (eg, intron 3, intron 6 or exon 6), qualitative or quantitative. (For intron 3 and intron 6 [including exon 6 if performed] analysis, use 81315). (If both intron 6 and exon 6 are analyzed, without intron 3, use one unit of 81316)

2025 CPT code 81315

PML/RARalpha,(t(15;17)), (promyelocytic leukemia/retinoic acid receptor alpha) (eg, promyelocytic leukemia) translocation analysis; common breakpoints (eg, intron 3 and intron 6), qualitative or quantitative

2025 CPT code 81275

KRAS (Kirsten rat sarcoma viral oncogene homolog) (eg, carcinoma) gene analysis; variants in exon 2 (eg, codons 12 and 13)

2025 CPT code 81380

HLA Class I typing, high resolution (ie, alleles or allele groups); one locus (eg, HLA-A, -B, or -C), each.

2025 CPT code 81402

Molecular pathology procedure, level 3.This includes analysis of >10 single nucleotide polymorphisms (SNPs), 2-10 methylated variants, 2-10 somatic variants, immunoglobulin and T-cell receptor gene rearrangements, duplication/deletion variants of one exon, loss of heterozygosity (LOH), and uniparental disomy (UPD).

2025 CPT code 81278

IGH@/BCL2 (t(14;18)) (eg, follicular lymphoma) translocation analysis, major breakpoint region (MBR) and minor cluster region (mcr) breakpoints, qualitative or quantitative.

2025 CPT code 81408

Molecular pathology procedure, Level 9 (eg, analysis of >50 exons in a single gene by DNA sequence analysis).

2025 CPT code 81258

HBA1/HBA2 (alpha globin 1 and alpha globin 2) gene analysis for known familial variants.

2025 CPT code 81163

BRCA1 and BRCA2 gene analysis; full sequence analysis.

2025 CPT code 81217

BRCA2 (BRCA2, DNA repair associated) (eg, hereditary breast and ovarian cancer) gene analysis; known familial variant

2025 CPT code 81255

HEXA (hexosaminidase A [alpha polypeptide]) (eg, Tay-Sachs disease) gene analysis, common variants (eg, 1278insTATC, 1421+1G>C, G269S).

2025 CPT code 81331

Methylation analysis of SNRPN and UBE3A genes (e.g., for Prader-Willi and/or Angelman syndromes).

2025 CPT code 81232

Analysis of common variants in the dihydropyrimidine dehydrogenase (DPYD) gene (e.g., *2A, *4, *5, *6) to assess drug metabolism, primarily for 5-fluorouracil (5-FU) and capecitabine.

2025 CPT code 81246

Analysis of FLT3 gene for tyrosine kinase domain (TKD) variants.

2025 CPT code 81361

Analysis of the HBB gene for common variants (e.g., HbS, HbC, HbE).

2025 CPT code 81194

Analysis of NTRK1, NTRK2, and NTRK3 gene translocations.

2025 CPT code 81179

ATXN2 (ataxin 2) (eg, spinocerebellar ataxia) gene analysis, evaluation to detect abnormal (eg, expanded) alleles

2025 CPT code 81381

High-resolution HLA Class I typing for a single allele or allele group.

2025 CPT code 81112

Analysis of the common alleles of Human Platelet Antigen 15 (HPA-15), also known as CD109 (CD109 molecule), using a blood or amniotic fluid specimen.

2025 CPT code 81370

HLA Class I and II typing, low resolution, molecular techniques.

2025 CPT code 81109

Analysis of common Human Platelet Antigen 5 (HPA-5) gene variants (e.g., HPA-5a/b [K505E]) using a patient specimen (e.g., blood).

2025 CPT code 81332

Analysis of common SERPINA1 gene variants (e.g., *S and *Z) associated with alpha-1-antitrypsin deficiency.

2025 CPT code 81345

Analysis of the telomerase reverse transcriptase (TERT) gene, focusing on targeted sequences like the promoter region.