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ALL MEDICAL CODES IN CATEGORY Multianalyte Assays with Algorithmic Analyses

2025 CPT code 81520

Oncology (breast), mRNA gene expression profiling by hybrid capture of 58 genes (50 content and 8 housekeeping), utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as a recurrence risk score.

2025 CPT code 81554

Pulmonary disease (idiopathic pulmonary fibrosis [IPF]), mRNA, gene expression analysis of 190 genes, utilizing transbronchial biopsies, diagnostic algorithm reported as categorical result (e.g., positive or negative for high probability of usual interstitial pneumonia [UIP]).

2025 CPT code 81540

Oncology (tumor of unknown origin), mRNA, gene expression profiling by real-time RT-PCR of 92 genes (87 content and 5 housekeeping) to classify tumor into main cancer type and subtype, utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as a probability of a predicted main cancer type and subtype.

2025 CPT code 81551

Oncology (prostate), promoter methylation profiling by real-time PCR of 3 genes (GSTP1, APC, RASSF1), utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as a likelihood of prostate cancer detection on repeat biopsy.

2025 CPT code 81509

Fetal congenital abnormalities, biochemical assays of three proteins (PAPP-A, hCG [any form], DIA), utilizing maternal serum, algorithm reported as a risk score.

2025 CPT code 81510

Multianalyte assay with algorithmic analysis; maternal serum screening for fetal chromosomal abnormalities.

2025 CPT code 81503

Oncology (ovarian), biochemical assays of five proteins (CA-125, apolipoprotein A1, beta-2 microglobulin, transferrin, and pre-albumin), utilizing serum, algorithm reported as a risk score.

2025 CPT code 81529

Oncology (cutaneous melanoma), mRNA, gene expression profiling by real-time RT-PCR of 31 genes (28 content and 3 housekeeping), utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as recurrence risk, including likelihood of sentinel lymph node metastasis.

2025 CPT code 81595

Cardiology (heart transplant), mRNA, gene expression profiling by real-time quantitative PCR of 20 genes (11 content and 9 housekeeping), utilizing subfraction of peripheral blood, algorithm reported as a rejection risk score.

2025 CPT code 81523

Oncology (breast), mRNA, next-generation sequencing gene expression profiling of 70 content genes and 31 housekeeping genes, utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as index related to risk to distant metastasis.

2025 CPT code 81536

This test predicts the response of gynecologic tumors to additional chemotherapy drugs, using a live tumor cell culture, DAPI staining, morphology, and a predictive algorithm.

2025 CPT code 81596

This test uses six biochemical assays (ALT, A2-macroglobulin, apolipoprotein A-1, total bilirubin, GGT, and haptoglobin) with a prognostic algorithm to generate scores for liver fibrosis and necroinflammatory activity in patients with chronic hepatitis C virus (HCV) infection.

2025 CPT code 81518

mRNA gene expression profiling for 11 genes with algorithmic analysis to determine metastatic recurrence risk and extended endocrine therapy benefit.

2025 CPT code 81514

Infectious disease, bacterial vaginosis and vaginitis, quantitative real-time amplification of DNA markers for Gardnerella vaginalis, Atopobium vaginae, Megasphaera type 1, BVAB-2, and Lactobacillus species (L. crispatus and L. jensenii), vaginal fluid, algorithm for bacterial vaginosis, includes detection of Trichomonas vaginalis and/or Candida species.

2025 CPT code 81517

Liver disease, analysis of 3 biomarkers (hyaluronic acid [HA], procollagen III amino terminal peptide [PIIINP], tissue inhibitor of metalloproteinase 1 [TIMP-1]), using immunoassays, utilizing serum, prognostic algorithm reported as a risk score and risk of liver fibrosis and liver-related clinical events within 5 years.

2025 CPT code 81493

Coronary artery disease (CAD) risk score determined via gene expression profiling by real-time RT-PCR of 23 genes using whole peripheral blood.

2025 CPT code 81508

Biochemical assays of two proteins (PAPP-A, hCG), utilizing maternal serum, algorithm reported as a risk score. Do not report with 84163, 84702.

2025 CPT code 81528

Colorectal cancer screening using a stool sample; detects DNA markers and fecal hemoglobin; algorithmic analysis reports a positive or negative result.

2025 CPT code 81512

This code represents a multianalyte assay with algorithmic analysis (MAAA) that uses results from multiple analytes and patient data to generate a risk score.It includes all analytical services and the algorithmic analysis.

2025 CPT code 81560

This CPT code represents a multianalyte assay with algorithmic analysis (MAAA) to assess the risk of allograft rejection in pediatric liver or small bowel transplant patients. The test measures CD154+ T-cytotoxic memory cells and uses an algorithm to generate a rejection risk score.

2025 CPT code 81525

Oncology (colon), mRNA, gene expression profiling by real-time RT-PCR of 12 genes (7 content and 5 housekeeping), utilizing formalin-fixed paraffin-embedded tissue, algorithm reported as a recurrence score.

2025 CPT code 81522

mRNA gene expression profiling by RT-PCR of 12 genes (8 content and 4 housekeeping) using formalin-fixed paraffin-embedded tissue; algorithm reported as recurrence risk score for breast cancer.

2025 CPT code 81539

Analyzes blood for total, free, and intact PSA, and hK2; algorithm reports probability score for high-grade prostate cancer.

2025 CPT code 81506

Algorithmic analysis of seven diabetes-related analytes (glucose, HbA1c, insulin, hs-CRP, adiponectin, ferritin, interleukin-2 receptor alpha) from serum or plasma to generate a risk score.

2025 CPT code 81546

Oncology (thyroid), mRNA, gene expression analysis of 10,196 genes, using fine needle aspirate; algorithm reported as a categorical result (e.g., benign or suspicious).

2025 CPT code 81490

Autoimmune (rheumatoid arthritis) analysis of 12 biomarkers using immunoassays, utilizing serum, prognostic algorithm reported as a disease activity score.

2025 CPT code 81519

Oncology (breast), mRNA, gene expression profiling by real-time RT-PCR of 21 genes, utilizing formalin-fixed paraffin embedded tissue, algorithm reported as recurrence score.

2025 CPT code 81511

Biochemical assays of four maternal serum analytes (AFP, uE3, hCG, DIA) with algorithmic analysis resulting in a risk score for fetal congenital abnormalities.