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BETA v.3.0

2025 CPT code 81374

HLA Class I typing, low resolution (eg, antigen equivalents); one antigen equivalent (eg, B*27), each

When testing for the presence or absence of more than two antigen equivalents at a locus, use 81373 for each locus tested. Procedures required prior to cell lysis (eg, microdissection) should be reported separately. Modifier 26 can be appended if only the interpretation and report are performed by a physician.

Modifier 26 can be used if only the professional component (interpretation and report) is performed by a physician.

Medical necessity is dependent upon the clinical indication for testing.Examples include:suspicion of HLA-associated diseases, organ transplantation matching, or other clinically relevant reasons.

In simple words: This test examines specific genes related to your immune system, called HLA genes.It helps determine the presence of certain variations in these genes, which can be associated with particular diseases or be important for organ transplants. This test doesn't look at every detail of the gene, but provides a general overview of a specific part of it, like identifying the "B*27" type.

This code represents the laboratory testing for low or intermediate resolution typing of a single HLA Class I antigen equivalent.It includes all analytical steps involved, from nucleic acid extraction to identification of the specific allele group. The test typically focuses on a single antigen equivalent of interest, often related to a specific condition.It's important to note that procedures prior to cell lysis, such as microdissection, are reported separately. While this test may be used for various purposes, it is commonly employed to identify HLA Class I antigen equivalents associated with specific disease states. For instance, it can be used to detect B*27, an allele group linked to ankylosing spondylitis.

Example 1: A patient with suspected ankylosing spondylitis needs HLA-B27 typing to confirm the diagnosis., A potential organ donor undergoes HLA typing for preliminary matching with recipients., A patient requires HLA typing to assess the risk of certain autoimmune diseases.

Documentation should include the specific HLA Class I antigen equivalent tested, the reason for testing (e.g., diagnostic, pre-transplant), and the interpretation of the results.

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