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BETA v.3.0

2025 CPT code 88166

Cytopathology, slides, cervical or vaginal (the Bethesda System); with manual screening and computer-assisted rescreening under physician supervision.

Use this code when both manual and computer-assisted screenings are performed on the same slide under physician supervision. Ensure the report uses the Bethesda System for reporting the results.

Medical necessity for this code depends on the patient’s symptoms, risk factors, and clinical presentation. Routine screening, follow-up of abnormal findings, and evaluation of specific symptoms can justify the use of this code.

The lab analyst performs the technical aspects of the procedure, including manual and computer-assisted screening. A physician supervises the process and interprets the results.

In simple words: This test analyzes cells collected from the cervix or vagina (like a Pap smear) to check for abnormalities. A lab technician first examines the cells under a microscope and then uses a computer program to double-check the results. The results are reported using a standardized system called the Bethesda System.

This code represents a cytopathology procedure for cervical or vaginal slides using the Bethesda System reporting method. It involves an initial manual screening of the slide followed by a computer-assisted rescreening, all performed under the supervision of a physician.This is typically used when the initial manual screening identifies an abnormality requiring further evaluation with computer assistance.

Example 1: A patient undergoes a routine Pap smear as part of an annual exam. The initial manual screening shows atypical cells. The slide is then rescreened using computer assistance to confirm the findings., A patient experiences vaginal bleeding and undergoes a Pap smear. The initial manual screening is inconclusive.A computer-assisted rescreening is performed to aid in the diagnosis., A patient with a family history of cervical cancer undergoes a Pap smear. The manual screening shows some abnormal cells, and the computer-assisted rescreening helps determine the severity of the abnormality.

Documentation should include the type of specimen collected, the method of preparation, the results of both manual and computer-assisted screenings, and the interpretation using the Bethesda System terminology.

** It is important to differentiate this code from other Pap smear codes based on the specific method of screening and reporting used.

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