2025 CPT code 81514

Infectious disease, bacterial vaginosis and vaginitis, quantitative real-time amplification of DNA markers for Gardnerella vaginalis, Atopobium vaginae, Megasphaera type 1, BVAB-2, and Lactobacillus species (L. crispatus and L. jensenii), vaginal fluid, algorithm for bacterial vaginosis, includes detection of Trichomonas vaginalis and/or Candida species.

Do not separately report component tests (e.g., individual assays for Gardnerella vaginalis or Candida) as they are included in the 81514 code.Use 81599 (Unlisted molecular pathology procedure) only when a specific MAAA code is not available.

Medical necessity for 81514 is established when a patient presents with signs or symptoms suggestive of bacterial vaginosis or other vaginal infections.This may include abnormal vaginal discharge, odor, itching, or discomfort.The test can also be medically necessary for screening during pregnancy or for evaluating recurrent vaginitis.

The laboratory analyst performs the technical aspects of the test, including specimen processing, DNA extraction and amplification, and algorithmic analysis of the results. They also report the findings, which may include a positive or negative result for BV and the presence of other organisms like Trichomonas vaginalis and Candida species.

In simple words: This test helps determine if you have bacterial vaginosis (BV) or other vaginal infections. It analyzes a sample of vaginal fluid to detect the DNA of bacteria associated with BV. It also checks for other infections like Trichomonas and Candida (yeast). The results are processed by a computer program to give a positive or negative result for BV.

This code represents a multianalyte assay with algorithmic analysis (MAAA) for infectious disease, specifically bacterial vaginosis and vaginitis. It involves quantitative real-time amplification of DNA markers for six bacterial species: Gardnerella vaginalis, Atopobium vaginae, Megasphaera type 1, Bacterial Vaginosis Associated Bacteria-2 (BVAB-2), and Lactobacillus species (L. crispatus and L. jensenii). The analysis utilizes vaginal-fluid specimens, and an algorithm is applied to report a positive or negative result for a high likelihood of bacterial vaginosis. The test also includes the separate detection of Trichomonas vaginalis and/or Candida species (C. albicans, C. tropicalis, C. parapsilosis, C. dubliniensis), Candida glabrata, and Candida krusei, when reported.

Example 1: A woman presents with abnormal vaginal discharge and odor.Her physician orders the 81514 test to determine if she has bacterial vaginosis and/or other vaginal infections like Trichomonas or Candida., A pregnant woman is being screened for bacterial vaginosis as part of her prenatal care, using code 81514., A patient with recurrent vaginitis undergoes testing using 81514 to identify the underlying cause and guide appropriate treatment.

Documentation should include the patient's symptoms, relevant medical history (including previous vaginal infections), the reason for ordering the test, and the results of the 81514 test.Any additional findings, such as the detection of Trichomonas vaginalis or Candida species, should also be documented.

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