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BETA v.3.0

2025 CPT code 86356

Quantitative analysis of mononuclear cell antigen (e.g., by flow cytometry), not otherwise specified, for each antigen.

Report 86356 once for each antigen tested. For example, if both CD45RA and CD45RO are analyzed, report 86356 twice. Ensure the documentation clearly specifies each antigen quantified separately.

Modifiers may be applicable to further specify the circumstances of the test. Refer to current CPT coding guidelines for appropriate modifier usage.

Medical necessity must be established by documenting the clinical rationale for the test. The documentation should clearly link the test to the patient's signs, symptoms, or diagnosis and explain how the test results will influence patient management.

The laboratory analyst performs the technical aspects of the test, including sample preparation, staining, and analysis using a flow cytometer.

IMPORTANT:Do not report 88187-88189 for interpretation of 86355, 86356, 86357, 86359, 86360, 86361, 86367. For unlisted cell types in an immunodeficiency panel, previously reported with 86586, now use 86356. If testing for CD45RA and CD45RO in an immunology study, consider code 86356 for cell counts. However, specific codes for total cell counts exist (e.g., 86359 for T cells). For flow cytometry panels in blood or lymphoid cancers, report the entire panel using 88184-+88185 (technical) and 88187-88189 (interpretation).

In simple words: This test measures the amount of specific markers (antigens) on the surface of your immune cells.It helps doctors diagnose and track conditions affecting your immune system.

This code represents the quantitative analysis of a specific mononuclear cell antigen, often using flow cytometry. The process involves staining cells with fluorescently marked antibodies that bind to specific antigens. These stained cells are passed through a flow cytometer, which uses a laser to identify and count cells based on their associated antigens.This test is crucial for diagnosing and monitoring various conditions, including blood disorders and immunodeficiencies, by identifying and quantifying specific cell populations.

Example 1: A patient with suspected leukemia undergoes flow cytometry to quantify different types of white blood cells, including specific antigens like CD45RA and CD45RO, to aid in diagnosis and classification of the disease., A patient with recurrent infections undergoes testing to quantify specific lymphocyte subsets, such as CD4 and CD8 T cells, to evaluate their immune function and diagnose potential immunodeficiencies., A patient undergoing treatment for lymphoma undergoes regular flow cytometry testing to monitor the effectiveness of therapy by tracking changes in specific cancer-associated antigens.

Documentation should include the specific antigen(s) tested, the method of analysis (e.g., flow cytometry), the clinical indication for the test, and the interpretation of the results.

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