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2025 CPT code 88274

Molecular cytogenetics; interphase in situ hybridization, analysis of 25-99 cells.

Follow CPT guidelines for pathology and laboratory services.Accurate cell count is crucial for appropriate code selection.

Modifiers may be applicable depending on the circumstances of service. Refer to the CPT manual and payer guidelines for appropriate modifier usage.

Medical necessity is established when the ordering physician documents a clinical indication for chromosomal analysis, such as suspicion of a specific genetic condition or cancer based on the patient's symptoms, family history, or other diagnostic findings.

A cytogenetic laboratory analyst performs the interphase FISH procedure.The ordering physician is responsible for interpreting the results in the context of the patient's clinical presentation and other diagnostic information.

IMPORTANT:For analysis of 100-300 cells, use CPT code 88275.Refer to CPT codes 88272-88275 for other cell count ranges and metaphase/interphase variations. For molecular pathology procedures not specifically listed, consider using CPT codes 81479 or 81599.If the test involves acetylcholinesterase, use 82013; for alpha-fetoprotein (serum or amniotic fluid), use 82105 or 82106; for laser microdissection of cells from a tissue sample, use 88380.

In simple words: This lab test looks at the chromosomes in a sample of your cells (like blood or tissue) using a special technique called FISH.It checks for problems with the chromosomes that might indicate a genetic condition or cancer. The test is done on 25-99 cells.

This CPT code encompasses the performance of a molecular cytogenetic test using interphase in situ hybridization (ISH), specifically analyzing 25 to 99 cells.The procedure involves applying fluorescently labeled DNA probes to a specimen (e.g., bone marrow, lymph nodes, blood, tumor tissue, amniotic fluid) to identify specific DNA sequences.The analysis determines the presence, absence, or position of the targeted DNA sequence, revealing potential chromosomal abnormalities.Formalin-fixed or paraffin-embedded samples, even those several months old, may be suitable. The method is advantageous for its speed and ability to analyze non-dividing cells.

Example 1: A patient presents with suspected Down syndrome.Interphase FISH analysis using a probe targeting chromosome 21 is performed on a blood sample to detect trisomy 21., A patient with leukemia undergoes bone marrow biopsy. Interphase FISH is used to detect specific chromosomal translocations associated with the diagnosis and prognosis of different leukemia subtypes., A pregnant woman undergoes amniocentesis. Interphase FISH is performed on fetal cells to rapidly screen for common chromosomal abnormalities, such as trisomy 13, 18, and 21.

* Patient demographics and relevant medical history.* Indication for testing (clinical suspicion).* Specimen type and collection date and time.* Results of the interphase FISH analysis, including the number of cells analyzed and the interpretation.* Physician's interpretation and clinical correlation of results.

** This code is used for interphase FISH analysis.The choice between this code and similar codes (88272, 88273, 88275) depends on the number of cells analyzed.Always consult the most up-to-date CPT manual and payer-specific guidelines for proper coding.

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