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BETA v.3.0

2025 CPT code 88313

Special stain including interpretation and report; Group II, all other (e.g., iron, trichrome), except stain for microorganisms, stains for enzyme constituents, or immunocytochemistry and immunohistochemistry.

Report one unit of 88313 for each special stain performed on each surgical pathology block, cytologic specimen, or hematologic smear. Do not report this code for routine stains like hematoxylin and eosin (H&E).

Modifiers may be applicable depending on the specific circumstances of the service provided.

Medical necessity is determined by the clinical indication for the special stain. The documentation should support the rationale for performing the special stain and its contribution to the diagnostic process.

The lab analyst performs all technical steps to stain the specimen slide. A qualified provider (typically a pathologist) interprets the slide and issues a report.

IMPORTANT:For immunocytochemistry and immunohistochemistry, use 88342. For special stains for microorganisms, use 88312. For histochemical stain on frozen tissue block, use 88314. Use 0757T in conjunction with 88313 when digitization of glass microscope slides is performed.

In simple words: A lab technician performs a special staining procedure on a tissue sample to help diagnose a medical condition.A doctor then examines the stained sample under a microscope and provides a report of their findings. This doesn't include stains for microorganisms, enzymes, or special immune-related stains.

This code represents the examination and interpretation of a special stain, excluding stains for microorganisms, enzyme constituents, or immunocytochemistry and immunohistochemistry. It includes the technical steps of staining the specimen slide with a special stain for Group II identification, interpretation of the results by a qualified provider, and a report of the findings. This code is used for each special stain performed on each surgical pathology block, cytologic specimen, or hematologic smear.

Example 1: A patient undergoes a biopsy of a skin lesion. To identify specific components within the tissue, a trichrome stain is performed on the specimen, and the results are interpreted by the pathologist., A patient has a bone marrow biopsy. An iron stain is performed to assess iron stores within the bone marrow, and the pathologist interprets the findings., A patient has a fine needle aspiration of a suspicious neck mass. A special stain is used to differentiate various cell types present in the specimen, and the results are interpreted by the pathologist.

Documentation should include the type of special stain performed, the specific specimen(s) stained, the interpretation of the stain results, and the corresponding report.

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