BETA v.3.0

2025 CPT code 89321

Semen analysis: presence and motility of sperm (if performed).

Refer to the latest CPT guidelines and payer-specific coding policies for proper use of this code.

Modifiers 59, 79, 90, 91, and others may be applicable depending on the circumstances of service. Consult the CPT manual and payer specific guidelines for appropriate modifier usage.

Medical necessity for this test is established when there is a clinical indication such as investigation of male infertility or post-vasectomy evaluation.The physician should document the clinical reasons for ordering the test and correlate the results with the patient's overall clinical picture.

The clinical responsibility lies with the laboratory analyst who performs the technical aspects of the semen analysis, including microscopic examination for sperm presence and motility assessment.

IMPORTANT:For Hyaluronan binding assay (HBA), use 89398.Code 89300 (Semen analysis; presence and motility of sperm including Huhner test) is not appropriate for post-vasectomy semen analysis as it includes the Huhner test, which is not typically performed in post-vasectomy analysis. Check payer policy to determine the use of G0027 (Semen analysis; presence and motility of sperm excluding Huhner).

In simple words: This lab test checks a semen sample to see if it contains sperm and if the sperm are able to move.The sample needs to be tested shortly after it's collected. This test might be done to see if a man is having trouble fathering a child or to check if a vasectomy was successful.

This CPT code reports a semen analysis to determine the presence and motility of sperm. The analysis involves microscopic examination of a semen specimen to assess sperm count and, if present, their motility.The specimen should be analyzed within one hour of collection. This test may be ordered to evaluate male infertility or to confirm the success of a vasectomy.

Example 1: A male patient presents for a post-vasectomy semen analysis 12 weeks after the procedure to verify its success.The lab performs a semen analysis (89321) to assess sperm presence and motility.The results confirm azoospermia (absence of sperm), indicating successful vasectomy., A couple experiencing infertility undergoes a comprehensive fertility workup. As part of the evaluation, the male partner provides a semen sample for analysis (89321).The results reveal oligospermia (low sperm count) and asthenospermia (poor sperm motility), contributing to the infertility diagnosis., A patient presents with symptoms suggestive of male infertility.The physician orders a semen analysis (89321). The laboratory findings show a normal sperm count but reduced sperm motility, suggesting a potential cause of infertility.

* Patient demographics and identification.* Date and time of specimen collection.* Method of specimen collection.* Time elapsed between collection and analysis.* Detailed microscopic findings (sperm count, motility, morphology, etc.).* Physician's order specifying the test.

** The laboratory performing the analysis must be CLIA certified.Payers may have specific requirements regarding the inclusion or exclusion of the Huhner test.

** Only Enterprise users with EHR integration can access case-specific answers. Click here to request access.