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2025 CPT code 0064U

Antibody, Treponema pallidum, total and rapid plasma reagin (RPR), immunoassay, qualitative.

This code (0064U) should only be used when both Syphilis Total and RPR are ordered and performed concurrently. If the tests are performed separately, use the appropriate individual codes (86780 and 86592). Do not report any other CPT codes with 0064U for the same analysis.

Medical necessity is established by the patient's symptoms, risk factors, or need to monitor treatment response for syphilis. Refer to specific payer guidelines for coverage details.

The lab analyst performs the immunoassay on a patient specimen, typically serum, to detect and quantify both treponemal and RPR antibodies. Results are used by clinicians to diagnose or manage syphilis treatment.

IMPORTANT:If tests are performed separately, use 86780 for treponemal antibody and 86592 for non-treponemal antibody (RPR).

In simple words: This test checks for syphilis by looking for two types of antibodies in a blood sample. It's a single test that gives more complete information than older methods that required two separate tests.

BioPlex™ 2200 Syphilis Total & RPR Assay, from Bio–Rad® Laboratories, a single immunoassay panel to evaluate both total treponemal antibody and non-treponemal antibody rapid plasma reagin (RPR) in a patient specimen to aid in syphilis diagnosis or treatment management.

Example 1: A patient presents with symptoms suggestive of syphilis. This test is ordered to provide a comprehensive assessment of both treponemal and RPR antibodies, aiding in diagnosis., A patient with a history of syphilis is being monitored for treatment response. This test is used to track changes in RPR antibody levels, which typically decline with successful treatment., A patient has a positive treponemal antibody test (86780). This test is then used as a confirmatory test, simultaneously providing RPR information for a more complete picture.

Documentation should include the reason for testing, the patient's signs and symptoms, and relevant medical history. The specimen type and date of collection should also be documented.

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