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2025 CPT code 81377

HLA Class II typing, low resolution (e.g., antigen equivalents); one antigen equivalent, each.

Refer to CPT guidelines for Molecular Pathology Procedures for detailed instructions.

Modifier 26 can be appended if only the interpretation and report are performed by a physician or other qualified healthcare professional.

Medical necessity is determined by the need for a transplant. HLA typing is necessary to find a compatible donor and increase the chances of a successful transplant.

The lab analyst performs all technical aspects of the test including nucleic acid extraction, amplification, and identification of the HLA Class II antigen.

IMPORTANT:When testing for the presence or absence of more than 2 antigen equivalents at a locus, use 81376 for each locus.

In simple words: This test helps determine the compatibility between a donor's and recipient's immune systems for transplants. It examines specific markers on your cells to see if a donor is a good match for you.

This code represents the laboratory testing for low or intermediate resolution typing of one HLA Class II antigen equivalent.The process involves identifying alleles without high specificity, often to the level of the two-digit HLA name (e.g., DRB1*15). While not restricted to specific conditions, this test is frequently used to identify HLA Class II antigen equivalents or alleles associated with particular disease states, such as DRB1*15 and multiple sclerosis.The testing includes all technical steps from nucleic acid extraction (via methods like cell lysis and digestion) to identification of the target HLA Class II antigen equivalent (using methods like amplification, hybridization with nucleic acid probes, or direct sequencing-based typing).

Example 1: A patient awaiting a kidney transplant needs HLA typing to identify compatible donors., A doctor orders HLA typing for a sibling of a patient with leukemia to determine compatibility for a bone marrow transplant., A patient diagnosed with multiple sclerosis undergoes HLA typing to check for the presence of specific HLA markers associated with the disease.

Documentation should include the reason for the HLA typing, the patient's medical history, and the method used for typing.

** Preparatory work like tissue selection (88363) or microdissection (88380) should be reported separately.Physician interpretation and report can be separately reported with G0452 and modifier 26 (for Medicare and some other payers).

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