2025 CPT code 81375
(Active) Effective Date: N/A Revision Date: N/A Laboratory - Molecular Pathology Procedures Pathology and Laboratory Feed
HLA Class II typing, low resolution (HLA-DRB1/3/4/5 and -DQB1).
Modifier 26 can be used to indicate that only the professional interpretation and report of the test were performed.
Medical necessity for 81375 is established when there's a clinical indication such as organ transplant evaluation, bone marrow transplant monitoring, or workup of autoimmune disease.The test should be medically appropriate considering other diagnostic tools available and should not be performed excessively without clinical justification.
The clinical responsibility lies with the laboratory analyst who performs all technical aspects, from nucleic acid extraction to HLA gene sequence identification.
In simple words: This lab test identifies certain genes (HLA Class II genes) that help the body recognize and fight off foreign invaders. The test uses advanced lab methods to check the genes without extremely precise identification. This test may help match organ or stem cell donors and recipients or diagnose certain autoimmune diseases.
This CPT code encompasses the technical component of low or intermediate-resolution Human Leukocyte Antigen (HLA) Class II typing for HLA-DRB1/3/4/5 and -DQB1 loci using molecular techniques.The procedure includes nucleic acid extraction (e.g., cell lysis, digestion), amplification, and identification of target HLA gene sequences (e.g., hybridization with probes, direct sequencing).The results provide allele identification without high specificity (e.g., not to the two-digit HLA name level).
Example 1: A patient is preparing for a kidney transplant.This test is ordered to determine the compatibility between the patient's HLA Class II alleles and those of the potential donor., A patient presents with symptoms suggestive of an autoimmune disease. HLA Class II typing is performed to identify specific alleles associated with particular autoimmune disorders., A bone marrow transplant recipient is monitored for graft-versus-host disease, which is sometimes assessed using HLA typing.
Detailed patient demographics, clinical indication for testing (e.g., transplant compatibility, autoimmune disease workup), sample type and collection date, and the HLA typing results with interpretation.
** This code represents low- or intermediate-resolution typing. Higher resolution typing would require different codes.