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2025 CPT code 81382

High-resolution HLA Class II typing for one locus.

Refer to CPT guidelines for molecular pathology procedures.

Modifier 26 may be appended if only interpretation and report are performed by a physician.Other modifiers may apply based on specific circumstances, such as the place of service or multiple loci tested.

Medical necessity is established by the ordering physician based on clinical indication (e.g., pre-transplant compatibility testing, diagnosis of autoimmune diseases, post-transplant monitoring).Payers may require supporting documentation to justify the test.

The lab analyst performs all technical aspects, from nucleic acid extraction to identifying HLA gene sequences. Physician interpretation may be separately reported with modifier 26 (if ordered and performed by a physician).

IMPORTANT:When only the presence or absence of a single allele or allele group is reported using high-resolution testing, use 81383. When high-resolution typing of any or all of the DRB3/4/5 genes is performed, treat as one locus. If HLA Class II typing is done using serology, use codes 86812-86821 instead.For more than one Class II locus, report one unit of 81382 per locus.Use 81378 and 81382 for HLA-A, -B, -C, -DRB1, and -DRB3/4/5, instead of 81379 and two units of 81382.Use 81382 when the high-resolution HLA Class II test involves more than two alleles or allele groups at a single locus.

In simple words: This lab test identifies specific genes (HLA Class II genes) that help the body distinguish its own cells from foreign invaders. It's a detailed test, providing precise information about these genes for things like organ or stem cell transplants, or diagnosing certain diseases.

This CPT code represents the performance of high-resolution typing for one HLA Class II locus (e.g., HLA-DRB1, -DRB3/4/5, -DQB1, -DQA1, -DPB1, or -DPA1).The procedure includes all analytical steps, from nucleic acid extraction (e.g., cell lysis, digestion) to identifying target HLA gene sequences (e.g., amplification, hybridization, direct sequencing). High-resolution typing identifies common and well-documented alleles with high specificity (e.g., 4-digit HLA name). For rare alleles, it might involve reporting a string of alleles or a National Bone Marrow Donor Program code.

Example 1: Pre-transplant evaluation to assess donor-recipient compatibility for a kidney transplant. High-resolution HLA Class II typing is crucial to minimize the risk of rejection., Diagnosis of a suspected autoimmune disease where certain HLA Class II alleles are associated with increased risk. The test helps confirm or rule out the genetic predisposition., Monitoring of a patient's immune system following a bone marrow transplant.Regular HLA typing helps track the engraftment of donor cells and detect any potential complications.

Detailed patient demographics, specimen type (e.g., blood, tissue), date and time of collection, indication for testing, and complete results of the HLA typing with interpretation (if applicable).

** This code is for high-resolution typing.If lower-resolution typing is performed, different codes should be used.Specific HLA loci tested should be clearly documented.

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